Abstract This study assessed the association between encapsulated nitrate product (ENP) and monensin (MON) to mitigate enteric methane (CH4) in vitro and possible effects on ruminal degradability, enteric fermentation characteristics, and microbial populations. Six treatments were used in randomized complete design in a 2×3 factorial arrangement with two levels of MON (0 and 2.08 mg/mL of buffered rumen fluid) and three levels of ENP (0, 1.5 and 3.0%). The substrate consisted of 50% Tifton-85 hay and 50% concentrate mixture (ground corn and soybean meal). ENP replaced soybean meal to achieve isonitrogenous diets (15% CP). No ENP×MON interaction was observed for any measured variable (P > 0.05) except for the relative abundance of F. succinogenes (P = 0.02) that linearly increased in diets with MON when ENP was added. The ENP addition decreased CH4 production (P < 0.01) without affecting (P > 0.05) truly degraded organic matter nor the relative abundance of methanogens. Hydrogen production was reduced with MON (P = 0.04) and linearly decreased with ENP inclusion (P = 0.02). We concluded that use of nitrate is a viable strategy for CH4 reduction, however, no additive effect of ENP and MON was observed for mitigating CH4 production.

Resumo O objetivo deste estudo foi avaliar os efeitos da infecção por Trichostrongylus colubriformis nos parâmetros hemato-bioquímicos, digestibilidade aparente dos nutrientes e balanço de nitrogênio de cordeiros Santa Inês. Dezoito cordeiros Santa Inês, de três meses de idade e castrados (16,9 ± 1,43 kg de peso corporal), foram distribuídos aleatoriamente em dois tratamentos experimentais: infectados com T. colubriformis (I, n = 9) e não infectados (U, n = 9). O grupo I recebeu um total de 45.000 larvas L3 de T. colubriformis (5.000 larvas infectantes, três vezes por semana, durante três semanas). Durante o período experimental, foram avaliadas as variáveis sanguíneas, digestibilidade e balanço de nitrogênio. Os cordeiros I apresentaram redução de eritrócitos, hemoglobina, hematócrito, volume corpuscular médio e proteínas totais, além de aumento de plaquetas e eosinófilos, quando comparados ao grupo U (p< 0,05). Contudo, exceto para proteínas totais, os valores estavam dentro do intervalo normal para a espécie. Além disso, a digestibilidade da matéria seca e da matéria orgânica foi menor no grupo I de cordeiros (p = 0,08). Os presentes achados destacam que a infecção por T. colubriformis teve potencial para prejudicar alguns parâmetros hemato-bioquímicos, bem como a digestibilidade aparente da dieta, o que poderia afetar a produtividade dos cordeiros.

Abstract This study aimed to evaluate the effects of Trichostrongylus colubriformis infection on the hemato-biochemical parameters, feed digestibility, and nitrogen balance in Santa Inês lambs. Eighteen three-month-old Santa Ines castrated male lambs (16.9 ± 1.43 kg of body weight) were randomly distributed in two experimental treatments: infected with T. colubriformis (I, n = 9) and uninfected (U, n = 9). The I group received a total of 45,000 L3 larvae of T. colubriformis (5,000 infective larvae, three times per week, for three weeks). During the experimental period, blood, feed digestibility, and nitrogen balance were evaluated. The I lambs showed a reduction in erythrocytes, hemoglobin, hematocrit, mean corpuscular volume, and total proteins, as well as an increase in platelets and eosinophils compared to those in the U group (p < 0.05). With the exception of total protein content, these values were within the normal range for the species. Furthermore, lower dry matter and organic matter digestibility were observed in the I lambs (p = 0.08). The present findings highlight that T. colubriformis infection has the potential to impair some hemato-biochemical parameters as well as feed digestibility in lambs, which could affect their productivity.

ABSTRACT: Crude glycerol is a major by-product of biodiesel production and is an economical additive feed for ruminants. However, residual methanol in crude glycerol can be harmful to animal health. Several methods exist for quantifying methanol residues in biodiesel, yet few have been described that identify the methanol level in crude glycerol. We propose a method for determining the methanol level in crude glycerol destined for animal feed. Crude glycerol was extracted from the headspace and quantified by gas chromatography using a flame ionization detector (GC-FID). The method was linear up to 0.5 % of methanol. The limits of detection (LOD) and quantification (LOQ) were 0.015 and 0.031 %, respectively. No significant matrix effects were observed. Precision was 2 % at the 0.05 and 0.5 % levels. The average percentage of recovery was 90 %. Three analyzed samples of crude glycerol had methanol residues of 0.027-7.802 %. Furthermore, this methanol quantification method may be externally or internally calibrated using a GC manual injector. A reduction of at least 20 % in running time was obtained with good resolution between the peaks. Thus, this method can be applied to determine the methanol level in crude glycerol according to the upper limits for animal feed (5,000 ppm) and for human consumption (150 ppm). Finally, this method is useful for the quality control of crude glycerol intended for use in animal feed, enabling the alternative use of this by-product to add value to the biodiesel production chain.

ABSTRACT: Gossypol easily pairs with lysine side chains and enzymes involved in the cellular growth process. The effect of gossypol (a compound present in cotton co-products) in ruminant metabolism and meat quality is not yet clear. This study was undertaken in order to evaluate the effects of cotton co-products in lamb muscle proteome. Twenty-four Santa Inês ram lambs, 5-months old (20.6 ± 1.9 kg), were randomly assigned to four treatments: control (without cottonseed), whole cottonseed, cottonseed meal and high oil cottonseed meal. At 95 days into the experiment, lambs were slaughtered and samples from Longissimus dorsi were collected. Proteins were extracted and analyzed by 2-D electrophoresis. Spots showing a significant effect from the treatment (the “treatment effect”) and present in more than 90 % of the samples were identified using mass spectrometry. Cotton co-products decreased the abundance of aldehyde and malate dehydrogenases, creatine kinase M-type and Adenosine triphosphate (ATP) synthase. They also increased four proteins related to muscle contraction. Thus, feeding cotton co-products to lambs changed the abundance of important muscle proteins. A cotton co-product diet induced a negative impact on the energy supply of muscle cells and, consequently, the abundance of ATP dependent proteins (contractile apparatus) increased, probably in order to offset and maintain muscle function. These proteomic changes can promote our understanding of alterations in the sensorial properties of meat due to cotton co-product diets in further investigations.

ABSTRACT: A reliable determination of endogenous phosphorus (P) excretion is required to measure P utilization in chickens accurately. The objective of this study was to investigate phosphorus (P) retention in broilers fed diets formulated with different feed ingredients. Sixty-four 15-d old broiler chicks were fed diets in which part of the dietary P was provided from dicalcium phosphate, maize, barley or soybean. Level of supplementation of each ingredient was calculated to provide two levels of total P (4.5 or 5.0 g kg−1 feed). Birds received a single injection of 3 MBq of 32P to determine endogenous P excretion using the isotope dilution technique. Four days after injection, blood and excreta were collected for analysis of inorganic and radioactive P. There were no differences among diets in total (p = 0.37) or endogenous (p = 0.65) P excretion or in P retention (p = 0.37) regardless of the supplemental feed material used in each diet. Daily P retention was increased (p = 0.004) as P intake increased, but the proportion of P ingested that was retained was not affected (p = 0.23) by the level of dietary P. The use of an isotopic tracer allows for accurate estimation of endogenous P in excreta, ranging from 0.24 to 0.42 mg P g−1 dry matter intake. The retention of P in growing chickens was not changed when 10-20 % of total P was provided by maize, barley or soybean.

ABSTRACT: The use of lambs from crossing of local with specialized sheep breeds for meat production may lead to higher meat deposition and a smaller fat amount in the carcass, with a more adequate nutritional profile for human consumption. This study investigated the performance, carcass and meat characteristics of lambs from the Morada Nova and Santa Inês locally adapted hair breeds and crosses between Dorper × Morada Nova and Dorper × Santa Inês slaughtered at approximately 35 kg. Morada Nova lambs showed lower (p < 0.05) performance compared to the other genetic groups resulting in a higher age at slaughter (p < 0.05). Crossbred Dorper × Santa Inês and Dorper × Morada Nova lambs showed better characteristics for meat production with better (p < 0.05) conformation as well as leg and carcass compacteness index. Dorper × Santa Inês have a fatty acid profile that is more suitable for human consumption than Dorper × Morada Nova lambs do (p < 0.05); however, both had lower (p < 0.05) intramuscular lipid content (as measured by ether extract levels from Longíssimus lumborum) compared with purebred lambs. Therefore, when the objective is to obtain lean meat, with improved nutritional profile to reduce risks of cardiovascular disease, the use of breeds and crosses that reduce animal slaughter age, such as the ½ Dorper × ½ Santa Inês crossbred, is an interesting alternative.

Resumo: O objetivo deste trabalho foi avaliar três espécies de gramíneas tropicais quanto à produção de metano (CH4) in vitro e à degradabilidade da matéria orgânica, para determinar as relações entre a qualidade nutritiva das gramíneas e a produção de CH4. O capim-colonião (Megathyrsus maximus), o capim-braquiarão (Urochloa brizantha) e o capim-decumbens (Urochloa decumbens) foram avaliados. O capim-braquiarão apresentou os maiores teores de matéria orgânica, fibra em detergente neutro, fibra em detergente ácido e lignina, e o menor teor de proteína bruta. O capim-decumbens apresentou os maiores teores de hemicelulose e de nitrogênio em fibra de detergente neutro, além do menor teor de celulose. O capim-colonião e o capim-decumbens apresentaram maior produção total de gases do que o braquiarão. Além disso, o capim-colonião apresentou maior produção de CH4, e o capim-braquiarão, menor degradabilidade verdadeira da matéria orgânica e reduzido fator de partição, em comparação ao capim-decumbens. Maior produção de CH4 foi verificada em casos em que os valores de hemicelulose e fator de partição foram menores. A qualidade nutricional e a produção de CH4 das forragens avaliadas podem ser utilizadas como parâmetros a serem empregados, que visem a sustentabilidade da produção de ruminantes.

Abstract: The objective of this work was to evaluate three tropical forage species for their in vitro methane (CH4) production and organic matter degradability, in order to determine the relationships between forage grass nutritive quality and CH4 production. Guinea grass (Megathyrsus maximus), palisade grass (Urochloa brizantha), and signal grass (Urochloa decumbens) were evaluated. Palisade grass showed the highest organic matter, neutral detergent fiber, acid detergent fiber, lignin, and lower-crude protein content. Signal grass had the highest values for hemicellulose and neutral detergent fiber-nitrogen, and the lowest-cellulose content. Guinea grass and signal grass showed a higher-total gas production than palisade grass. Besides, Guinea grass showed an increased CH4 production, and palisade grass showed lower value for truly degraded organic matter, and reduced partitioning factor, in comparison to signal grass. An increased CH4 production was observed in cases of lower hemicellulose and paratitioning factor. The nutritive value and CH4 production of forages may be employed as parameters, aiming at the sustainability of ruminant production.

RESUMO: A adubação com Selênio (Se) em pastagem para a biofortificação de alimentos de origem animal tem sido realizada em solos com baixo Se. O objetivo foi avaliar o aumento no conteúdo de Se na biomassa de Brachiaria spp. com aplicação de ureia revestida com Se. O experimento foi conduzido em casa de vegetação em Latossolo Amarelo. O delineamento foi em blocos casualizados com quatro repetições em esquema fatorial com dois cortes e seis doses de Se (0, 10, 20, 40, 80 e 160gha-1). A Brachiaria brizantha demonstrou ausência de mudanças em altas e perigosas doses de Se sem alterar a atividade da enzima antioxidante glutationa peroxidase. Com isso, concluiu-se que seu enriquecimento com ureia revestida é obtida com 34,5gha-1 de Se.

ABSTRACT: Selenium (Se) fertilization in grazing to biofortification of animal products have been carried out in low Se soils. The objective of this study was to increase the Se content in the biomass of Brachiaria spp.with urea coated with Se. The experiment was performed in a typical Hapludox soil under greenhouse conditions. A completely randomized block design with four replicates in a factorial structure with two cuts and six Se doses (0, 10, 20, 40, 80, and 160gha-1) was used. The Brachiaria brizantha demonstrated the absence of changes in higher harmful doses without modifying the activity of glutathione peroxidase enzyme. We concluded that enrichment of Brachiaria brizantha with coated urea is obtained with 34.5gha-1of Se.

ABSTRACT Cottonseed coproducts contain gossypol which can have specific effects upon the male reproductive system. We evaluated the reproductive development of male lambs close to puberty fed on three cotton coproducts. Twenty-four 5-month old male lambs received four diets: 20 % of dry matter intake of whole cottonseed (WCS), high oil cottonseed meal (CSC), cottonseed meal (CSM), and a control group (CTL) without any cottonseed coproducts. Free gossypol intake was 16.32, 6.98, 5.47 and 0 mg kg−1 BW−1 d−1 for WCS, CSC, CSM and CTL, respectively. Every 15 days, the animals were weighted and serum and semen samples were collected. After 95 days, testis samples were collected for analysis under light and transmission electron microscopes. The CTL group had higher testosterone concentrations than CSC at the end of the trial and lower total sperm defects, higher mass movement and higher scores for seminiferous epithelium than other treatments. The WCS and CSC groups showed higher levels of segmental aplasia lesion in sperm than other diets, which showed that high levels of gossypol led to higher occurrence of this lesion. Cottonseed coproducts had a negative impact on the reproductive system of pubertal lambs regardless of gossypol concentration. Therefore, the use of cottonseed coproducts to feed lambs earmarked for reproduction is not safe.

RESUMO O objetivo deste trabalho foi avaliar o uso de nitrato de cálcio em substituição ao farelo de soja em dieta de ruminantes sobre a mitigação do metano entérico visando determinar os custos com a dieta do animal e os custos marginais de abatimento de carbono. O estudo foi realizado em duas fases, na primeira foi avaliado o potencial de mitigação de metano do nitrato por produção de gases in vitro. Os dados de mitigação de metano foram utilizados na segunda fase, que simulou uma propriedade leiteira com três sistemas de alimentação controle, sem nitrato, e sistemas com inclusão de 1,5 e 3% de nitrato na dieta. Foram utilizadas planilhas de cálculo para avaliar os custos e receitas de cada sistema com vendas de crédito de carbono resultantes da redução de metano ao final de 10 anos. O valor presente líquido (VPL) e a taxa interna de retorno (TIR) foram os índices utilizados na avaliação financeira. A venda de crédito de carbono não alterou o VPL, no entanto a inclusão de nitrato a 1,5 e 3% na dieta animal mostrou-se economicamente viável, pois reduziu o valor das dietas (R$ 5,89 e 5,81/vaca/dia, respectivamente) em comparação com a dieta controle (R$ 6,13 /vaca/dia), além de contribuir para redução de gases de efeito estufa. Portanto, a suplementação de nitrato,como fonte de nitrogênio não protéico, em dietas para ruminantes, além de reduz a produção de metano possibilitou uma sensível redução no custo variável da atividade.

SUMMARY The objective of this work was to evaluate the calcium nitrate utilization in replacing to the soybean meal for ruminants diets under the mitigation of enteric methane aiming to determine the animal diet cost and the marginal costs for carbon rebates. The study was conducted in two stages, the first stage was in vitro gas production were performed in order to measure the enteric methane mitigation potential of the nitrate. The methane mitigation data were used in the second stage, which simulated a dairy farm with three feed systems: control system without nitrate, and systems with inclusion of 1.5 to 3% nitrate in the diet. The spreadsheets were used to evaluate the costs and revenues of each system resulting carbon credit sales of methane reduction in 10 years. The net present value (NPV) and the internal rate of return (IRR) were the indexes used in the financial evaluation.The carbon credit sale did not change the NPV significantly, however the inclusion of nitrate to 1.5 and 3% in animal feed showed economically feasible, because it reduced the diet value (R$5.89 e R$5.81/cow/day, respectively) in comparison to control diet (R$6.13/cow/day) besides contributing to the reduction and greenhouse gases. Therefore, the nitrate supplementation beside reduced the methane production, has the positive effect on the variable cost of the activity.

Objetivou-se, na condução deste trabalho, a avaliação das silagens de capim-elefante aditivadas com tortas de nabo forrageiro, pinhão manso e tremoço pela técnica de produção de gás. O experimento foi desenvolvido no Laboratório de Nutrição Animal do Centro de Energia Nuclear na Agricultura da Universidade de São Paulo (LANA/CENA/USP). Como doadores de líquido de rúmen, foram utilizados 2 ovinos da raça Santa Inês, machos, adultos, castrados e providos de cânula ruminal permanente. A alimentação dos animais doadores foi constituída de forragem de gramínea cultivada e uma suplementação, ao final do dia, com feno de Tifton, concentrado comercial e sal mineral à vontade. Os substratos foram secos a 60ºC, moídos em moinho do tipo Willey, provido de peneira com perfurações de 2 mm. Os gases produzidos durante os diferentes períodos de fermentação (0, 4, 8, 12, 24, 36, 48, 72 e 96 h) foram medidos com um transducer - medidor de pressão.O experimento foi instalado segundo um delineamento de blocos ao acaso em que os tratamentos foram arranjados em um esquema de parcelas subdivididas no tempo. Os maiores valores de produção de gás observados para os tratamentos em que adicionou-se torta de tremoço quando comparados com as outras tortas, decorreu do fato da torta de tremoço apresentar menor teor de fibras, propiciando assim, uma maior fermentação ruminal e, consequentemente, maior produção de gás em relação a outros alimentos com maior proporção de carboidratos estruturais (parede celular).As taxas de degradação da fração solúvel da matéria seca foi menor para NF 8% e PM 11% em relação às outras silagens estudadas. Foram encontradas diferenças significativas para as TNF, TPM e TT, nos diferentes níveis, em relação ao volume de gases em 96 h de incubação (P<0,05). As silagens contendo torta de tremoço apresentaram maior produção de gases quando comparadas Às outras tortas. Em todos os tratamentos, exceto naqueles em que adicionou-se TT, houve diminuição (P<0,05) nos valores médios de degradabilidade da matéria seca às 96 horas, à medida que aumentou-se o nível de inclusão das tortas. As silagens de capim-elefante adicionadas de tortas de nabo forrageiro ou tremoço, nos diferentes níveis, apresentaram maiores taxas de degradação e maiores produções de gases que as adicionadas de torta de pinhão manso.

The objective of this work was to evaluate elephant grass silages with forage radish, Jatropha and lupine cakes as additives by the gas production technique. The experiment was developed in the Animal Nutrition Laboratory of the Center of Nuclear Energy in Agriculture (Center Centro de Energia Nuclear na Agricultura) of São Paulo University (LANA/CENA/USP). As donors of rumen liquid, two sheep of the Santa Ines breed, males, adults and fitted with permanent rumen cannula were used. The donating animals' feeding consisted of cultivated grass forage and a supplementation ate the end of day with Tifton hay, commercial concentrate and mineral salt at will. The substrates were dried at 60ºC, ground in Willey type mill fitted with a sieve with perforations of 2 mm. The gases produced during the different fermentation periods (0, 4, 8, 12, 24, 36, 48, 72 and 96 h) were measured with a transducer - pressure measurer. The experiment was set up according to a randomized block design in which the treatments were arranged in a split plot in time. The greatest values of gas production were found for the treatments where lupine cake was added when compared with the other cakes. That originated from the fact that lupine cake presented lower fiber content, enabling increased rumen fermentation and, hence, higher gas production in relation to other feeds with a greater ratio of structural carbohydrates (cell wall). The degradation rate of the soluble fraction of dry matter was lower for 8% TNF and 11% TPM in relation to the other silages studied. Significant differences were found for the TNF, TPM and TT at the different levels relative to the amount of gas in 96 h of incubation (P<0.05). In all the treatments, except in those TT was added, there was decrease (P<0.05) in the average degradability values of dry matter at 96 hours as the level of inclusion of the cakes was increased. The elephant grass silages added with forage radish and lupine cakes at the different levels presented higher rates of degradation and higher gas production than those added with Jatropha.

O objetivo deste trabalho foi avaliar o potencial do extrato de acácia (EA) para o controle de T. colubriformis em ovinos. Os cordeiros foram alocados em três grupos de seis animais. Dois grupos foram experimentalmente infectados com larvas infectantes de T. colubriformis, sendo um grupo mantido como controle não infectado. Trinta dias pós-infecção, os animais infectados do grupo tratado foram suplementados com EA. Durante e após o tratamento os valores de contagem de ovos por grama de fezes (OPG), número de larvas de terceiro estágio por grama de fezes (LPG), número de OPG eliminado por animal durante o período de tratamento, viabilidade dos ovos e carga parasitária foram determinados. Os animais do grupo não infectado permaneceram com número de OPG igual a zero durante todo o período experimental. A média do número de OPG total eliminado por grupo, somatória durante os 10 dias de tratamento, foi menor nos cordeiros tratados com EA (P<0,05). Os valores de viabilidade dos ovos de helmintos foram menores (P<0,05) nos dias 4, 7 e 10 pós-tratamento (p.t.). O número de LPG foi menor (P<0,05), nos dias 10 e 14p.t., no grupo tratado. O EA demonstrou potencial para sua utilização no controle de T. colubriformis em ovinos.

The aim of this study was to evaluate the potential uses of acacia extract (AE) on Trichostrongylus colubriformis control in sheep. The lambs were allocated in three groups of six animals. Two groups were experimentally infected with third-stage larvae of T. colubriformis and one was remained as non-infected control. Thirty days post-infection, the infected animals from treated group were supplemented with AE. During and after the treatment, the values of faecal eggs counts (FEC), number of third-stage larvae recovered per gram of faeces (LPG), number of FEC accumulated per animal during the treatment period, nematode egg viability, and worm burden were determined. Animals from non-infected group did not presented FEC values, during the experimental period. The average of total accumulated amount of FEC output per group, during the 10 days of the treatment was lower in AE treated lambs (P<0.05). The values of egg viability were statistically reduced (P<0.05) on days 4, 7 and 10 post treatment (p.t.). The number of LPG was lower (P<0.05), at the days 10 and 14p.t., in treated animals. AE showed potential to be used on the prophylaxis of gastrointestinal nematode infections in sheep.

Com a introdução de óleo vegetal na cadeia produtiva do biocombustível no Brasil, é esperado o aumento da demanda por plantas oleaginosas e oferta de fibras vegetais resultantes da extração do óleo. Considerando a alimentação animal como elo entre a produção de biodiesel e a pecuária, propomos o estudo da utilização de subprodutos destes empreendimentos na alimentação visando aumentar a produtividade e diminuir a emissão de gases de efeito estufa pelos animais, gerando créditos de carbono e atendendo ao interesse da iniciativa privada. A técnica de produção de gases in vitro tem sido utilizada no LANA-CENA/USP com a finalidade de estudar o efeito de alimentos que possuem metabólitos secundários bioativos, na fermentação ruminal e degradabilidade da matéria orgânica (MOVD), bem como, no estudo da produção de metano. Resultados recentes mostraram que a substituição total do farelo de soja por tortas de algodão, dendê, mamona e pinhão manso proporcionou menor produção total de gases; e que a produção de metano foi significativamente afetada pela inclusão das tortas, sendo que o farelo de soja apresentou produção de metano de 15,3 mL/g MOVD. Diversos trabalhos mostram que as tortas e farelos apresentam características nutricionais adequadas para inclusão na dieta de ruminantes, entretanto, estudos criteriosos desses materiais enquanto matérias-primas para ração animal são necessários para avaliar possíveis efeitos deletérios devido à presença de metabólitos bioativos. Através de análises de cromatografia de alta resolução, podem ser identificadas essas substâncias que, em condições tropicais podem não apresentar a mesma toxicidade. A introdução de tortas com elevado teor de gordura nas dietas de ruminantes pode auxiliar na mitigação de metano entérico, e a produção de algumas oleaginosas pode contribuir com o seqüestro de carbono pelos solos de Cerrado na recuperação de pastagens, reduzindo a necessidade de desmatamentos.

With the introduction of vegetable oil in the bio-fuel productive chain in Brazil, the increase in the demand of oleaginous plants is expected as well as the offer of the resulting vegetable fibers from the extraction of the oil. Considering the animal feeding as link between the biodiesel production and the livestock, we propose the study of the use of by-products of these enterprises in the feeding system seeking to increase the productivity and to reduce the emission of greenhouse gases from the animals, generating carbon credits and assisting to the interest of the private initiative. The in vitro gas technique has been used at LANA-CENA/USP with the purpose of studying the effect of feeds containing secondary bioactive metabolites upon the ruminal fermentation and organic matter degradability (MOVD), as well as, in the study of methane production. The total substitution of the soybean meal for meals of cotton seed, palm oil, castor oil plant and jatropha provided less total gas production and the production of methane was affected significantly by the inclusion of the meals, with soybean meal presenting methane production of 15.3 mL/g MOVD. Literature data also show that meals from biodiesel production present appropriate nutritional characteristics for inclusion in the diet of ruminant, however, discerning studies of those materials while raw materials for animal ration are necessary to evaluate possible harmful effects due to the presence of bioactive metabolites. Through analyses of high resolution chromatography, those metabolites may be identified. The introduction of meals with high fat content in the diets of ruminant can aid in the mitigation of enteric methane, and the production of some oleaginous plants can contribute with the sequestering of carbon for the Cerrado soils in the recovery of pastures, reducing the need of deforestations

Quatro éguas sem raça definida (idade e peso corporal médios de seis anos e 400 kg) foram distribuídas em delineamento experimental em quadrado latino para se avaliar o valor nutritivo e o estudo cinético do trato digestivo de grãos secos ou ensilados de sorgo de baixo e alto conteúdos de tanino na alimentação de eqüinos. Os tratamentos consistiram de dietas contendo dois híbridos de grãos de sorgo (baixo e alto níveis de tanino) e dois métodos de conservação (secos e ensilados). As dietas foram isoprotéicas (12,4% PB), com ingestão diária de MS estabelecida em 1,5% PV (relação feno:concentrado de 50: 50). Os parâmetros de trânsito gastrintestinal avaliados foram: k1 (taxa de passagem pelo intestino grosso), k2 (taxa de passagem pelo estômago), TT (tempo de trânsito), TMR (tempo médio de retenção) e TMRT (tempo médio de retenção total). Os tratamentos não afetaram os coeficientes de digestibilidade aparente (CDa) da MS e do amido, cujos valores médios foram 54,04 e 98,91%, respectivamente. Verificou-se efeito benéfico da ensilagem dos grãos de sorgo de alto conteúdo de tanino sobre a digestibilidade da PB e FDN. A CDa da PB e FDN para a dieta contendo grãos secos de sorgo de alto teor de tanino foi de 49,76 e 32,20% e para as dietas com grãos de sorgo de baixo conteúdo de tanino (seco ou ensilado) e grãos ensilados de sorgo de alto teor de tanino foi de 65,63 e 43,32%, respectivamente. Obteve-se somente efeito do método de conservação dos grãos de sorgo (secos vs ensilados) sobre o TMR, em que o valor para as dietas com silagens de grãos ensilados e secos foi, respectivamente, de 40,08 e 37,9h. Concluiu-se que os grãos de sorgo secos de alto teor de tanino não devem ser usados como principal grão energético nos concentrados para eqüinos, por diminuírem a digestibilidade da proteína e fibra.

Four crosbred mares averaging six years and 400 kg BW were allotted to latin square design. The objective was to evaluate the nutritive values and kinetics of digestive tract of dry grains or high-moisture grains silage of sorghum with low and high tannin in the feeding of equines. The treatments consisted of diets containing two hybrids of sorghum grains (low and high tannin) and to methods of conservation (dry an ensiled). Diets were formulated to be isoprotein (12.4% CP) with daily DM intake established in 1.5% BW (hay to concentrate ratio of 50:50). The parameters of gastrointestinal transit were: k1 (passage rate in the large intestine); k2 (passage rate in the stomach); TT (transit time); MRT (mean retention time) and TMRT (mean total retention time). No effect treatment was observed for aCD of DM and starch, whose means values were 54.04 and 98.91%, respectively. A benefic effect of the ensiling of high tannin and high-moisture sorghum grains on digestibility of CP and NDF. The aCD of CP and NDF for diets containing high tannin and dry grains of sorghum was of 49.76% and 32.20% and for diets containing of low tannin sorghum grains (dry and ensiled) and high tannin and high-moisture sorghum grains was of 65.63 and 43.32%, respectively. There was effect only of the conservation method of the sorghum grains (dry versus ensiled) on the TMR, where the value for the diets with silages of high-moisture grains and dry grains was, respectively, of 40.08 and 37.9h. In conclusion, high tannin sorghum dry grains should not be used as the main grain in energetic concentrates for horses, because they reduced the digestion of protein and fiber.

Cinco éguas mestiças (idade e peso corporal médios de seis anos e 480 kg PV) foram distribuídas em delineamento experimental em quadrado latino para se e avaliar a utilização de dietas contendo grãos de triticale (secos ou ensilados) em substituição ao milho na alimentação de eqüinos, por meio do ensaio de avaliação do trânsito gastrintestinal e da digestibilidade. Os tratamentos consistiram de três níveis de grãos de triticale em substituição aos grãos de milho (0, 50 e 100%) e duas formas de conservação dos grãos de triticale (secos ou ensilados). As dietas foram isoprotéicas (12,5% PB), com ingestão diária de MS pelos animais de 2,0% PV (relação volumoso : concentrado de 50:50). Os coeficientes de digestibilidade aparente (CDa) da MS, MO, PB e FDN foram determinados indiretamente. Os parâmetros de trânsito gastrintestinal avaliados foram: k1 (taxa de passagem pelo intestino grosso), k2 (taxa de passagem pelo estômago), TT (tempo de trânsito), TMR (tempo médio de retenção) e TMRT (tempo médio de retenção total). Não houve diferença para os CDa da MS, MO, PB e FDN entre as dietas experimentais, observando-se valores médios de 64,31; 65,14; 74,13 e 57,25%, respectivamente. Considerando-se a cinética das fases sólida e líquida, notou-se efeito somente para k2 na fase sólida da digesta, cujo valor nas rações contendo 100% de triticale (seco ou ensilado) foi de 19,63%/h e na dieta controle (0% de triticale), de 23,72%/h. Observou-se efeito linear crescente para o TT na fase sólida da digesta, com a elevação dos níveis de substituição dos grãos de milho pelos grãos de triticale ensilados. Concluiu-se que os concentrados para eqüinos podem ser formulados com grãos de triticale secos ou ensilados em substituição total ao milho. A inclusão de grãos de triticale na alimentação desta espécie animal promoveu trânsito lento da fase sólida da digesta.

Five crossbred mares (averaging six years and 480 kg BW) were allotted to latin square experimental design. The objective was to evaluate the utilization of diets containing triticale grains (dry or ensiled) in substitution of corn in the feeding of equine feeding, using assay of gastrointestinal transit and digestibility. The treatments consisted of three levels of triticale grains in substitution of corn grains (0, 50 and 100%), and two conservation forms of triticale grains (dry or ensiled). The diets were isoproteic (12.5% CP), with aDM intake by the animals of 2,0% BW (forage to concentrate 50:50). The apparent coefficients of digestibility (aCD) of the DM, OM, CP and NDF were determined by indirect method. The parameters of the gastrointestinal transit were: k1 (passage rate in the large intestine); k2 (passage rate in the stomach); TT (transit time); TMR (mean time retention) and TMRT (mean total retention time). No difference was observed for aCD of DM, OM, CP and NDF among the experimental diets, with means values of 64.31, 65.14, 74.13 and 57.25%, respectively. Considering the kinetics of the solid and liquid phases, it was found effect only to the k2 for the solid phase of digesta, whose values in the diets containing 100% triticale (dry or ensiled) was of 19.63%/h and the control diet of 23.72%/h. An increasing linear effect was observed for TT in the solid phase of digesta, as the levels of substitution of corn grain by ensiled triticale grain increased. In conclusion, the concentrates for equines may be formulated with dry or ensiled triticale grains in total substitution of corn grains. The inclusion of the triticale grains in the equine feeding promoted slow transit of the digesta.